BioFactory systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up of cell culture applications such as production of vaccines, monoclonal antibodies, or pharmaceuticals. The BioFactory is a versatile and easy-to-use system for medium to large scale research, or commercial production with reduced contamination risk. The NEST BioFactory™ allows for the closed transfer of liquid, meaning it can directly transfer the culture medium, or cells, to a cell factory or a bioreactor through a connecting tube, in a sterile environment, thus greatly reducing the risk of exogenous pollution in the drug development and production process.